NanoQuantum Products
Example ICy and BrCy Near-Infrared Halo Dyes
| Dye Name |
|---|
| ICy-86 |
| ICy-90 |
| ICy-97 |
| ICy105 |
| BrCy106 |
| BrCy-10P-107 (BrCy107) |
None of these dyes contain metals
Alternate binding chemistry can potentially be prepared as a special request. COOH and NHS esters are generally available.
NQS has also developed other dyes that may be available for specific reporter or photosensitizer requirements. Help us understand your special requirements.
Spectral Data
Spectral data of free dyes characterize their behavior when bound to low-molecular-weight biomolecules such as amino acids, peptides, and oligonucleotides while the data for dye-IgG conjugates characterize behavior of the dyes when bound to high-molecular-weight biomolecules such as proteins and antibodies.
Spectral and physical characteristics of the example product dyes1
| Dye Name | Conjugate (D/P)2 |
Solvent System3 |
Absorption max. [nm] |
Extinction Coefficient (ε) [M–1⋅cm–1] |
Emission max. [nm] |
Quantum Yield (ΦF) [%] |
|---|---|---|---|---|---|---|
| Cy 7 | — | PB 7.4 | 747 | 200000 | 776 | 13 |
| Cy 7 | IgG (1.0) | PB 7.4 | ~750 | — | ~776 | 4 |
| ICy-86 | — | PB 7.4 | 721, 760 | 100000 | 786 | 0.6 |
| ICy-86 | — | MeOH | 765 | 200000 | 795 | 44 |
| ICy-86 | IgG (1.0) | PB 7.4 | 783 | — | 796 | 0.4 |
| ICy-90 | — | PB 7.4 | 751 | 270 | 778 | 27 |
| ICy-90 | IgG (1.0) | PB 7.4 | 757 | — | 778 | 6 |
| ICy-97 | — | MeOH | 764 | 200000 | 795 | 44 |
| ICy-105 | — | PB 7.4 | 750 | 227300 | 779 | 15 |
| ICy-105 | IgG (1.0) | PB 7.4 | 755 | — | 777 | 5 |
| BrCy-106 | — | PB 7.4 | 747 | 265500 | 775 | 18 |
| BrCy-106 | IgG (1.0) | PB 7.4 | 751 | 775 | 8 | |
| BrCy-10P-107 | — | PB 7.4 | 751 | 238000 | 779 | 20 |
| BrCy-10P-107 | IgG (1.0) | PB 7.4 | 754 | — | 779 | 9 |
1 The best reporters are highlighted with yellow.
2 D/P — dye-to-protein ratio
3 PB 7.4 — phosphate buffer pH 7.4
Relative Photostability
Fig. 1. Quantum yield of dye-IgG conjugates vs. dye-to-protein ratio in phosphate buffer (pH 7.4)
Fig. 2. Brightness vs D/P of dye-IgG conjugates when excited at the peak absorption
vs. dye-to-protein ratio (D/P) in phosphate buffer (pH 7.4)
The above data characterizes the photochemical behavior of selected NQS dyes bound to high-molecular-weight biomolecules such as proteins and antibodies using Cy 7 as a reference for comparison.
Cy 7 was selected as a reference because it functions in a similar wavelength range and is well known.
Example Photometric Characteristics of Selected Fluorescent Reporters
